Multi-Objective Multiplex PCR Design Version 3.0 (April 19, 2011) Help   Contact   Disclaimer	USER INFORMATION Name: Institution: Email: (required)   Solutions will be mailed to this address.
PRIMER SELECTION CRITERIA   Min Max Length bp %GC % Tm C Product bp Product Diff bp New! 3'/5' Dist bp Base Repeat     3'Tail ΔG   kCal/mol Max Tm Diff   C Align Score     Match     Mismatch     Gap Penalty     BLAT Filter? bp ProdSz Primer Pairs   (500 max)	SEQUENCES Assay name: (optional) File: Manual Entry: Test Samples Please cite: Rachlin J., Ding CM., Cantor C., and Kasif S.(2005). muPlex: A Multi-Objective Approach to Multiplex PCR Assay Design. Nucleic Acids Research. 33(Web Server Issue):W544-W547. --- Paste FASTA-formatted sequences below. --- Data Format: Sequence Only Sequence Brackets ...[...]... SNP Brackets [C/G] SNP IUPAC Ambiguity Code Mask Regions: NNNN or XXXX Lowercase atgc Target Plex: Algorithm: 1 solution (fastest) Limited tradeoffs Deep search (slowest)
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